TABLE 3.
Quantification of gold particles in the cytoplasm and cell wall-capsule of C. neoformans cells as determined by immunogold labeling
| Area of cell | Strain | Labelinga with MAb (no. of gold particles):
|
|||||
|---|---|---|---|---|---|---|---|
| 2D10 | 12A1 | 13F1 | 18B7 | 5C11 | Ricin 45 | ||
| Cytoplasm | B-3501 | 10.8 ± 8.5* | 1.6 ± 1.5 | 5.0 ± 4.4* | 1.6 ± 2.6 | 1.3 ± 1.8 | 0.8 ± 0.8 |
| B-4131 | 6.6 ± 6.0 | 1.2 ± 1.3 | 16.8 ± 6.3 | 0.0 ± 0.0 | 1.2 ± 2.2 | 0.8 ± 1.3 | |
| C536 | 19.8 ± 13.3* | 2.0 ± 2.3 | 5.0 ± 3.9 | 12.8 ± 4.9** | 0.6 ± 0.9 | 1.2 ± 2.2 | |
| Cell wall-capsule | B-3501 | 621.4 ± 401.0* | 7.8 ± 4.1* | 11.6 ± 3.6* | 309.2 ± 239.4 | 1.3 ± 2.2 | 0.2 ± 0.4 |
| B-4131 | 0.2 ± 0.4** | 2.0 ± 1.9 | 5.8 ± 1.6 | 3.8 ± 1.9** | 0.4 ± 0.9 | 0.0 ± 0.0 | |
| C536 | 5.0 ± 3.5** | 5.3 ± 1.3 | 9.0 ± 4.4 | 2.4 ± 2.5** | 1.8 ± 2.2 | 2.0 ± 2.0 | |
a
*, significant compared to 5C11; **, significant compared to B-3501.