TABLE 1.
Tryptic peptides of BoMac cytoskeletal proteins sequenced by mass spectrometry
Band | Most significant match | NCBI accession no. | Scorea | Peptides matched |
---|---|---|---|---|
1 | Alpha actinin | NP_068695 | 1,478 | ETTDTDTADQVIASFK plus 25 others |
2 | Vimentin | NP_776349 | 647 | DGQVINETSQHHDDLE plus 19 others |
3 | Cytokeratin 8 | P05786 | 505 | LEGLTDEINFYR plus 14 others |
4 | Actin | ATBOG | 792 | LCYVALDFEQEMATAASSSSLEK plus 16 others |
5 | Cytokeratin 19 | P08728 | 700 | QSSSTSSFGGMGGGMR plus 20 others |
The score is −10 times the log of P, where P is the probability that the observed match is a random event. Individual ions scores greater than 49 indicate identity or extensive homology (P < 0.05).