TABLE 1.
Possible functions of the proteins encoded by SrfA-dependent genesa
cDNA (gene) | GenBank accession no. | Product | Closest homologa | Gene expression patternb |
---|---|---|---|---|
Stress response | ||||
SLK452 | AY386221 | Catalase B | 1, spores | |
SSF584 | AY392429 | Peroxinectin | 1 | |
SLA632 | AY392430 | Heat shock protein 88 | 2 | |
SSB695 | AU037272 | Unknown | Low-temperature or -salt response (46) | 1 |
SSG693 | AY392431 | Unknown | DNA ligase (29) | 1 |
SSE445 | AY392432 | Unknown | DNA helicase (39) | 1 |
SLF664 | AY392438 | Unknown | DNA repair protein RAD50 (19) | 1, spores |
Cell signaling | ||||
SSK576 | AY392433 | Phospholipase C | 2, spores | |
SSE393 | AY392434 | Unknown | 5′ AMP-activated gamma subunit (27) | 2 |
Cytoskeleton and spore coat | ||||
SSF455 | D37981 | Cofilin B | 2, spores | |
SLB616 | X54452 | SpiAc | 1, spores | |
SSK268/SLJ453 | AY392441 | Unknown | Blackjack protein, microtubule associated (19) | 2, spores |
sigD | AY387647 | Unknownc | Spore coat proteins (28) | 1, spores |
Vesicle trafficking | ||||
SSM796 | AY392436 | Unknown | Synaptobrevin (40) | 2 |
SSB611 | AY392437 | Unknown | Mitochondrial carrier protein RIM (31) | 1 |
Cell adhesion | ||||
SSJ826 | AY392438 | Unknown | Tenascin X (31) | 1, stalks |
SSG726 | AY392439 | Unknown | P-selectin (24) | 1, stalks |
Metabolism | ||||
SLE765 | AY387644 | SigA malic enzymec | 2, spores | |
SSA535 | AY392440 | 3-Oxoacyl-acyl carrier | 2, stalks | |
SSJ666 | AY392442 | Unknown | Alkaline dihydroceramidase (22) | 2 |
Other proteins | ||||
SLA429 | AY392443 | Unknown | endotoxin (23) | 2 |
SSB379 | AY392444 | Unknownc | RNA-binding proteins (33) | 1, spores |
sigB | AY387645 | Unknownc | GP63 metalloproteinase (27) | 1, spores |
sigC | AY387646 | Phg1bc | 2 |
The percentage of amino acid identity is given in parentheses.
Expression pattern 1 indicates genes that are detected exclusively at late developmental stages while expression pattern 2 indicates genes also detected at earlier stages.
Genes previously identified as SrfA dependent (3). The genes coding for SigD, SigC (phg1b), and SigB were not present on the microarrays.