FIG. 1.

Regulation of NRG1 and NRG2 RNA levels in response to carbon source. Cultures of wild-type (WT) and snf1Δ strains (MCY4702 and MCY4758) were grown in YEP containing high (2%) glucose (Hi) and were shifted to low (0.05%) glucose (Lo) for 1 h. Wild-type cells were also grown in 2% glycerol-2% ethanol (GE); snf1Δ cells do not grow in a nonfermentable carbon source. RNA was prepared and subjected to Northern blot analysis. Blots were hybridized with probes specific for NRG1, NRG2, and the loading control, U3 RNA. Autoradiograms are shown. The panel at the right shows a shorter exposure for two lanes.