FIG. 2.

Regulation of Nrg1 and Nrg2 protein levels in response to carbon source. Wild-type (WT) and mutant strains of the indicated genotype (Table 1) expressed Nrg1-HA or Nrg2-HA from the chromosomal locus (+) or expressed the wild-type untagged proteins (−). Cultures were grown in YEP containing high (2%) glucose (Hi) and were shifted to low (0.05%) glucose (Lo) for 1 h or were grown in 2% glycerol-2% ethanol (GE). For the experiments shown in panels C and D, the shift to low glucose was for 3 h. Proteins were resolved by SDS-12% polyacrylamide gel electrophoresis, and proteins were blotted and detected with anti-HA antibody 12CA5 (A and B) or Babco HA.11 (C and D). Panel D shows two blots from different experiments. Arrows indicate the Nrg-HA proteins.