TABLE 2.

Transcriptional activation by LexA-Nrg1 in the ssn6Δ mutant^a

^aReporter plasmids contained the lacZ gene under control of the GAL1 promoter with UAS_G deleted (pLR1Δ1) (31) or replaced by 6 LexA binding sites (pSH18-18).

^bStrains MCY1974 (ssn6Δ) and MCY829 (wild type) were transformed with reporters containing 0 or 6 LexA binding sites and plasmids expressing LexA-Nrg1, LexA-Nrg2, or LexA alone (pV43, pV44, or pEG202).

^cCultures were grown to mid-log phase in selective SC plus 2% glucose (Hi); glucose-grown cells were also shifted to 0.05% glucose (Lo) for 3 h, as indicated.

^dβ-Galactosidase activity was assayed in permeabilized cells and expressed in Miller units. Values are averages for the results for 4 to 6 transformants. For values >1, standard errors were <15%. Values for LexA alone were comparable in high- and low-glucose conditions.