Figures 3.

(a–c); Quantitative evaluation of epi-fluorescent images of Tg(fli:EGFP) zebrafish embryos showing inhibitory effect of MK-886 on LTB4-induced angiogesis under normoxic condition. Histograms represent the angiogenic effects of LTB4 (0.1 µM), a selective agonist of PPARα on emergence of primary sprouts (fig a), ISV development (fig b) and DLAV development (fig c) at 28 hpf in the presence or absence of MK-886 (0.5 µM). Data are expressed as mean (±SEM) of total number of primary sprout (fig a) or the ratio between completely formed ISV (fig b) or DLAV (fig c) to the total number of ISV. A two way analysis of variance (ANOVA) was used to compare means of each group. *** p<0.001; *p<0.05