Figures 4.

a–c: cQuantitative evaluation of epi-fluorescent images of Tg(fli:EGFP) zebrafish embryos showing involvement of adenosine receptor in angiogenesis under normoxic or hypoxic condition. Histograms represent the effect of NECA (10 µM), a non selective agonist of adenosine receptor on emergence of primary sprout (ISV) (fig a), ISV development (fig b) and DLAV development (fig c) at 28 hpf in the presence of MRS-1706 (10 nM), a selective antagonist of A_2B adenosine receptor. Data are expressed as mean (±SEM) of total number of primary sprout (fig a) or the ratio between completely formed ISV (fig b) or DLAV (fig c) to the total number of ISV. A two way analysis of variance (ANOVA) was used to compare means of each group. *** p<0.001; **p<0.01; *p<0.05