FIG. 1.

The majority of the NDC1 gene is required for its function. NDC1 encodes a protein of 655 amino acids, and the structure of NDC1 includes seven predicted transmembrane domains, represented as white bars. The various NDC1 truncations are shown below the full-length NDC1. The ability of the NDC1 truncations to complement an NDC1 null strain (func.) was tested by the competence of ndc1Δ::KanMX/pALR10-NDC1 cells (strain 2709) to grow on 5-FOA plates in the presence of each of the seven NDC1 truncation plasmids. −, cells containing the NDC1 truncation construct cannot grow in the absence of the wild-type NDC1 plasmid; +, cells containing the NDC1 truncation construct can grow in the absence of the wild-type NDC1 plasmid. The subcellular localization (local.) of the various NDC1 truncations was determined by IF microscopy (see Materials and Methods). −, NDC1 truncations are not localized to the SPBs or NPCs; +, the NDC1 truncation is localized correctly to the SPBs and NPCs.