FIG. 4.

ndc1-39 exhibits genetic interaction with nic96-1. Suspensions of nic96-1 (strain 2252), ndc1-39 (strain 2710), and ndc1-39 nic96-1 (strains 3538 and 3539) cells were plated at an optical density at 600 nm of 3.0 in fivefold serial dilutions on plates containing synthetic medium lacking leucine and were grown at the permissive temperature of 23°C (left), at 30°C (middle), or at 34.5°C (right) for 3 days. Two strains of ndc1-39 nic96-1 mutants are shown.