FIG. 5.

ndc1-39 cells have normal nuclear protein import at the restrictive temperature. NDC1 (strain 2568/pJON280; first row), ndc1-39 (strain 3446/pJON280; second and third rows), and prp20-1 (strain 2925/pJON280; fourth row) (21) cells were grown at 35°C for 5 h in raffinose-containing medium that lacked uracil. Galactose was then added to induce the expression of the H2B1-GFP reporter (31) for 2 h, and cells were processed for fluorescence microscopy. DNA staining is shown in the left column, and GFP staining is shown in the right column. Bar, 1 μm.