FIG. 6.

ndc1-39 cells have normal NPC distribution at the restrictive temperature. NDC1 (strain 3345; first row) and ndc1-39 (strain 3346; second row) cells grown in YPD medium were shifted to 35°C for 3 h (left column) or 4 h (right column), whereas nup133Δ (strain 1258; third row) cells were grown at 23°C throughout the experiment. The cells were then processed for fluorescence microscopy. Two images of nup133Δ cells at the beginning of the experiment (0 h) are shown. DNA is shown in blue and Nup49p-GFP is shown in green. Each image shown was projected from five consecutive planes taken at 0.1-μm intervals along the z axis that have been deconvolved. Bar, 1 μm.