TABLE 1.
Yeast strains
| Strain | Relevant genotype | Reference or source |
|---|---|---|
| 1258 | MATanup133Δ::HIS3 nup49-1::URA3-nup49ΔGLFG::GFP-S65T-TRP1 ade2-1::ADE2-URA3 (SWY828) | 7 |
| 1264 | MATα NDC1 | 5 |
| 2252 | MATa nic96::HIS3 + pUN100-LEU2-nic96-1 | 44 |
| 2568 | MATaNDC1 | This study |
| 2709 | MATα ndc1Δ::KanMX + pALR10-NDC1 | This study |
| 2710 | MATandc1Δ::KanMX::ndc1-39-TRP1 | This study |
| 2804 | MATaSPC42-GFP-KanMX4 LYS2 | This study |
| 2925 | MATα prp20-1 leu2 trp 1 ura3 (PSY713) | This study |
| 3244 | MATa/α ndc1Δ::KanMX::ndc1-39-TRP1/ndc1Δ::KanMX::ndc1-39-TRP1 GAL10-GFP-S65T-nup49-URA3/GAL10-GFP-S65T-nup49-URA3 + pSW240 (pRS423-NUP49) | 21 |
| 3245 | MATa/α GAL10-GFP-S65T-nup49-URA3/GAL10-GFP-S65T-nup49-URA3 + pSW240 | This study; 6 |
| 3299 | MATa/α ndc1-1/ndc1-1 GAL10-GFP-S65T-nup49-URA3/GAL10-GFP-S65T-nup49-URA3 lys2-801/+ trp1-1/+ +pSW240 | This study; 6 |
| 3345 | MATa/α nup49-1::URA3-nup49ΔGLFG::GFP-S65T-TRP1/nup49-1::URA3-nup49ΔGLFG::GFP-S65T-TRP1 | This study; 6 |
| 3346 | MATa/α ndcIΔ::KanMX::ndc1-39-TRP1/ndc1Δ::KanMX::ndc1-39-TRP1 nup49-1::URA3-nup49ΔGLFG::GFP-S65T-TRP1/nup49-1::URA3-nup49ΔGLFG::GFP-S65T-TRP1 | This study; 6 |
| 3446 | MATαndc1Δ::KanMX::ndc1-39-TRP1 | This study |
| 3447 | MATandc1Δ::KanMX::ndc1-39-TRP1 SPC42-GFP-KanMX4 | This study |
| 3448 | MATa/andc1Δ::KanMX::ndc1-39-TRP1/ndc1Δ::KanMX::ndc1-39-TRP1 SPC42-GFP-KanMX4/SPC42-GFP-KanMX4 | This study |
| 3451 | MATandc1Δ::KanMX SPC42-GFP-KanMX4 +pRS314-NDC1-3xmyc | This study |
| 3452 | MATandc1Δ::KanMX SPC42-GFP-KanMX4 +pRS314-ndc1-39-3xmyc | This study |
| 3538 | MATα ndc1Δ::KanMX::ndc1-39-TRP1 nic96::HIS3 + pUN100-LEU2-nic96-1 | This study |
| 3539 | MATα ndc1Δ::KanMX::ndc1-39-TRP1 nic96::HIS3 + pUN100-LEU2-nic96-1 | This study |