TABLE 2.
Primers
| Primer | Sequencea | Restric- tion site |
|---|---|---|
| LAE1 | ATCTACCTTTCTGGGCTCCTGG | |
| LAE2 | CGTGAAGAACTTGGCGTTGTAG | |
| LA2 | GACGAGCTCGTGGAACAGTGGAAGGAAC | SacI |
| LA3 | GCGAAGCTTATGAACCGCATCAACCGA | HindIII |
| OEF | GCTGTGAAGCTTTGTACCCTGTTTCGCC | HindIII |
| OER | GATTTGAAGCTTTGCTGGCATGGAACGG | HindIII |
| MT1 | ATGCTGAAGCTTGGAAACTGGGAAAGGGGTC | HindIII |
| GFP2 | TGACGAATTCTCTTAATGGTTTCCTAGCCTG | EcoRI |
| GFP31 | TGCGGAATTCATGAGCAAGGGCGAGGAA | EcoRI |
| GFP4 | GGATGCCTCGAGTTTGTACAGCTCGTCCATGC | XhoI |
| GFP5 | AAGCAGCTCGAGTAAGAGCAAAAGGCGACCAC | XhoI |
| GF1 | CTAGCGAAGCTTGCCACCATGAGCAAGGGCG | HindIII |
| GF2 | CGGCGAATTCCTTGTACAGCTCGTCCATGC | EcoRI |
| GF3 | TTTGGAATTCGTTTCGCCGCTGATGTTTGAG | EcoRI |
| FUM1 | GCGCACTTCTTTGTTTTCCCCT | |
| FUM2 | CATCGGAATTCTTTCTTGAGCGGCC | EcoRI |
| FUM3 | TACCAGGATCCAAAACCTCTCGCCA | BamHI |
| FUM4 | CATGACGGTAACTAAGGATTTGG |
a
Underlined sequences show the placement of restriction sites.