Depletion of external Na+ by P. sorbitophila and U. maydis. K+-starved cells of S. cerevisiae, P. sorbitophila, and U. maydis were suspended in testing buffer, with Na+ or Na+ plus K+ added to the suspension, and the variations of the external concentration of Na+ were recorded at intervals in the absence of K+ (closed circles) or in the presence of 50 μM K+ (open circles). The cell densities (dry weight, in milligrams per milliliter) in the experiments were as follows: S. cerevisiae, 0.7; P. sorbitophila, 0.9; and U. maydis, 0.7.