FIG. 3.

Differential effect of ybp1Δ and ybh1Δ on H₂O₂-induced nuclear recruitment of Yap1p. An isogenic series of strains containing the indicated alleles of YBH1 and YBP1 were transformed with a low-copy-number plasmid expressing a GFP-Yap1p fusion protein under the control of the wild-type (wt) YAP1 promoter (4). Transformants were grown to mid-log phase and challenged with H₂O₂ for the indicated lengths of time. Cells were then visualized by fluorescence microscopy and filters appropriate for detection of GFP or DAPI fluorescence.