FIG. 7.

Overexpression of Ybh1p but not Ybp1p can suppress the H₂O₂ sensitivity of a gpx3Δ strain. A strain lacking the GPX3 gene (gpx3Δ) was transformed with plasmids expressing different antioxidant genes. A vector plasmid corresponding to pRS315 containing the PGK promoter (vector) was used to drive the expression of full-length GPX3 (PGK-GPX3), YBP1 (PGK-YBP1), or YBH1 (PGK-YBH1). A high-copy-number plasmid containing the YAP1 gene (2μm YAP1, YEp351-YAP1) (39) was also used to produce high levels of Yap1p. Transformants were analyzed by spot test analysis on H₂O₂ gradient plates as described previously (4).