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Expression of Ybp1p but not Ybh1p can restore H2O2-induced nuclear accumulation of Yap1p. A mutant strain lacking both YBP1 and YBH1 was transformed with a low-copy-number vector containing the PGK promoter driving expression of Ybp1p, Ybh1p, or no additional yeast sequence along with a GFP-Yap1p-containing reporter construct. Transformants were grown to mid-log phase, treated with H2O2 for 20 min, and visualized by fluorescence microscopy.
