FIG. 9.

Constitutive nuclear localization of a mutant form of Yap1p is independent of Ybp1p function. (A) GFP-C629A Yap1p expressed from a centromeric plasmid was introduced into a yap1Δ ybp1Δ mutant strain. Transformants were grown to mid-log phase, stained with DAPI, and visualized by fluorescence microscopy. (B) Low-copy-number plasmids containing either the wild-type or C629A YAP1 alleles were introduced into a yap1Δ ybp1Δ mutant strain along with an empty vector plasmid (pRS315). Transformants were grown to mid-log phase and analyzed for H₂O₂ and diamide tolerance as described above by a gradient plate assay.