FIG. 1.
Effect of protease inhibitors on DC-induced degradation of PrPSc derived from ScGT1-1 cells. (A) Immunoblot showing levels of PK-resistant PrP in ScGT1-1 cells, ScGT1-1 cells after exposure to DC, and DC combined with leupeptin treatment and (B) DC (green) after 24 h of coculture with ScGT1-1 cells in the presence of leupeptin. Note the presence of intracellular PrPSc (red). (C) Levels of PK-resistant PrP from ScGT1-1 cells exposed to DC for 48 h and after treatment of these cocultured cells with E-64c, E-64d, leupeptin, phosphoramidon, pepstatin, and aprotinin for 48 h. (D) Levels of PrPSc after incubation of ScGT1-1 homogenate (H) with DC in the absence and presence of leupeptin at 0 and 48 h. The duplicates represent different cultures.