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Effect of pH on PrPSc degradation. Immunoblot showing levels of PrPSc after incubation of ScGT1-1 lysates at pH 5.5 and 7.8 with the addition of DC lysates and DC lysates combined with leupeptin for 1 h. Note the decrease in PrP intensity at pH 5.5 after incubation with DC lysates, which was not seen when leupeptin was added. The duplicates represent different samples.
