FIG. 3.
Effect of PrPC RNA interference on the occurrence of PrPC and PrPSc in ScGT1-1 cells. (A) ScGT1-1 cells were treated with Cy3-labeled siRNAs (red), showing transfection of the majority of the cells; cell nuclei are shown in blue. (B) Immunoblot showing reduction of PrPC and loss of PK-resistant PrP 7 days after PrPC siRNA treatment of ScGT1-1 cells. a, untreated cells; b, PrPC siRNA-treated cells. −, non-PK-treated samples; +, PK-treated samples. (C and D) PrP immunofluorescence of untreated (C) and PrPC siRNA-treated (D) ScGT1-1 cells. The cells in both panels C and D were treated with guanidine thiocyanate prior to immunostaining to expose PrPSc. Bars, 20 μm.