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Presence of PrPSc in ScGT1-1 cell cultures after treatment with protease inhibitors. (A) Immunoblot showing the presence of PK-resistant PrP in ScGT1-1 cell cultures untreated or treated with E-64c, E-64d, leupeptin, aprotinin, pepstatin, and phosphoramidon for 48 h. (B and C) PrP immunofluorescence with Fab D13 of (B) untreated and (C) leupeptin-treated ScGT1-1 cells. Cells were exposed to guanidine thiocyanate prior to immunostaining. Bars, 20 μm.
