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Expression of Bcl-2, Bcl-xL, Bax, and cyclin D2 in primary ATL cells treated with FR901228. Leukemic cells obtained from patients with ATL were treated with (+) or without (−) 5-ng/ml FR901228 for 24 h. Cell lysates (50 μg of protein per lane) were fractionated on SDS-polyacrylamide (10 or 12%) gels and analyzed by Western blotting with antibodies directed against each protein. Comparable protein loading was verified with an antibody specific for the housekeeping gene product actin.
