FIG. 6.

Inhibition of constitutive NF-κB and AP-1 activities in HTLV-1-infected T-cell lines and primary ATL cells treated with FR901228. HTLV-1-infected T-cell lines (A) and primary ATL cells (B) were treated with (+) or without (−) 5-ng/ml FR901228 and assessed for NF-κB and AP-1 binding. After 24 h, nuclear proteins were extracted and EMSA was performed with NF-κB-, AP-1-, or Oct-1-specific radiolabeled oligonucleotide probes. Specificity of NF-κB and AP-1 binding was determined by using antibodies to the NF-κB components p50, p65, c-Rel, and p52 (C) and AP-1 components c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB, and JunD (D), resulting in supershift.