Figure 5.

Boldine prevents lipid peroxidation in mesangial cells grown in high glucose and pro-inflammatory cytokines. Graph showing levels of TBARS (MDA) in the culture medium of mesangial cells cultured for 48 h, under normal glucose (white bar), high glucose + cytokines (TNF-α (10 ng/mL) and IL-1β (10 ng/mL)) (black bar), or high glucose + cytokines treated with 100 μM boldine during the last 24 h (grey bar). Values are expressed as μmol of MDA per g of protein in the culture medium. Each bar represents the mean value ± SEM of 4 independent experiments. **P ≤ 0.01, using one-way ANOVA test followed by Bonferroni's post hoc test.