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Reciprocal growth competition assays between WT (triangles) and ENF-resistant HIV-1NL4-3 (circles), inoculated at a ratio of 1:1. Recombinant viruses carrying ENF resistance mutations (selected during in vitro passage experiments) at gp41 codons 37 (A and B), 38 (C and D), or 36 and 38 (E and F) linked to a hisD (A, C, and E) or PLAP (B, D, and F) sequence tag in nef were competed against recombinants carrying WT gp41 linked to PLAP (A, C, and E) or hisD (B, D, and F).
