Figure 5.

Expression profiling of the microarray data for genes involved in interferon-related signal transduction pathways following administration of CAWS to B6 and DBA/2 mice. Mosaic tiles and hierarchical clustering of microarray data are shown for the genes involved in interferon signaling (A), the role of protein kinase R (Pkr) in interferon induction and antiviral response (B), acute phase response signaling (C), and acute myeloid leukemia signaling (D). B6 and DBA/2 samples were clustered using a hierarchical clustering program (Spearman) to discover gene-to-gene relationships. Irf1, Eif2ak2, Trl, Lbp, Cebpb, Sfpil1, and Csf1r are highlighted by black arrows, Cfb and C3 by turquoise, Ifng by red and Irf9 by green arrows. (E) Intensity gradients indicate the mean value of the expression level (log2 ratio): blue (down-regulation) and crimson (upregulation) are shown compared to the average value at 0 w after CAWS administration (gray). (F) Scatter plots of the highlighted genes in log of signal intensity at 2 w (left panel) or 4 w (right panel) versus 0 w following CAWS administration in DBA/2 mice; data are plotted along the vertical and horizontal axes (arbitrary unit: a.u.), respectively. Highlighted genes are Ifng (red font), C3 (turquoise font), Cfb (turquoise font), and Irf9 (green font).