FIG. 1.
(A and B) Digital micrographs of plaques or singly infected cells in J cells infected with HSV-1(JMP) (A) or HSV-1(MP) (B). Infection was revealed by immunostaining with PAb directed against gM (1:1,500). The micrographs in panels A and B are at the same magnification. (C) Growth of HSV-1(JMP) and HSV-1(MP) and recombinants R-gDJMP and R-gDMP in J cells and BHK-tk− cells. J cells were infected with 10 PFU/cell, whereas BHK-tk− cells were infected with 0.01 PFU/cell, according to the titer determined in Vero cells. Viruses were absorbed to cells for 90 min at 37°C; excess virus was removed, and the remaining infectivity was inactivated with a pH 3 citrate buffer wash. Cells were frozen at 24 and 48 h after infection. The titer of progeny virus was determined by plaque assay in Vero cells.