FIG. 2.

Neutralization of HSV-1(JMP) infectivity by MAb HD-1. Aliquots of HSV-1(JMP) virions were incubated with the indicated amounts of purified IgGs from MAb HD-1 or an irrelevant MAb (MAb 6E2 directed against HHV-6) for 1 h at 37°C prior to infection of J cells grown in 24-well dishes. Infected cells were immunostained 48 h later with anti-gM PAb (1:1,500), followed by anti-rabbit antibodies conjugated to alkaline phosphatase (1:3,000). Data are expressed as percentages of the number of plaques obtained in infected cells not exposed to antibodies.