FIG. 4.

Infectivity inhibition by soluble receptors. Aliquots of HSV-1(JMP) virions (100 PFU/well) were incubated with the indicated nanomolar concentrations of soluble N3(VCC)-Fc (N3-Fc), N1(VCC)-Fc (N1-Fc), the V domain of nectin2-Fc [N2(V)-Fc], or BSA for 1 h at 37°C prior to infection of J cells (grown in 24-well dishes). Infectious events (plaques or singly infected cells) were detected by immunostaining with PAb directed against gM (1:1,500), followed by anti-rabbit antibodies conjugated to alkaline phosphatase (1:3,000). Data are expressed as a percentage of the number of plaques or of infectious events, relative to the values obtained in infected cells not exposed to soluble receptors. The values are averages from three replicate samples.