FIG. 6.
(A) FACS analysis of the binding of gDJMP or wt gD to J cells. gDJMP and wt gD (500 ng) [affinity purified from lysates of HSV-1(JMP)-infected BHK cells] were reacted with J cells for 1 h at 4°C. Binding was revealed by incubation with MAb H170 (1:1,000) directed against the N terminus of gD, followed by incubation with an FITC-conjugated anti-mouse antibody (1:100). The negative control (leftmost line) represents reactivity to MAb H170 and an FITC-conjugated anti-mouse antibody. (B) ELISA binding of soluble N1(VCC)-Fc (N1-Fc), N2(VCC)-Fc (N2-Fc), N3(VCC)-Fc (N3-Fc), nectin4Δ-Fc (N4-Fc), and CD28-Fc to gDJMP. Ninety-six wells were coated with gDJMP affinity purified from HSV-1(JMP)-infected BHK cells (80 ng/well) and were reacted with the indicated soluble receptors. Binding was revealed by adding anti-human Fc antibodies coupled to peroxidase (1:6,000) and then o-phenylenediamine and reading the optical density (O.D.) at 490 nm.