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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: Nat Commun. 2013;4:2834. doi: 10.1038/ncomms3834

Figure 2. STAT6 and PPARγ Activity are Normal in Tsc1Δ/Δ BMDMs.

Figure 2

a. Immunoblot analysis of lysates from Tsc1fl/fl and Tsc1Δ/Δ BMDMs stimulated with IL-4 for 5–60min. b. STAT6 luciferase reporter assay in Tsc1fl/fl and Tsc1Δ/Δ BMDMs. Data shown as fold induction of firefly luciferase activity normalized to renilla luciferase for IL- 4 treatment relative to untreated sample (n=2 experiments performed in duplicate). c. Gene expression and immunoblots for PPARγ and PPARδ in Tsc1fl/fl and Tsc1Δ/Δ BMDMs after treatment with IL-4 for 24h. Gene expression data is shown as mean ± SEM (n=3). d. Expression of PPARγ-dependent genes in Tsc1fl/fl and Tsc1Δ/Δ BMDMs treated with IL-4 in the presence or absence of troglitazone for 24h. DMSO vehicle was used as control, ( n=3). *p<0.05 for IL-4 treated Tsc1fl/fl and Tsc1Δ/Δ, **p<0.01, ***p<0.001 for IL-4 versus IL-4+Tro. e. PPAR luciferase reporter assay in Tsc1fl/fl and Tsc1Δ/Δ BMDMs. Data shown as fold induction of firefly luciferase activity normalized to renilla luciferase for IL-4 or troglitazone treatment relative to untreated sample (representative of 3 experiments performed in triplicate). Graphs are shown as mean ± SEM. P-values determined using Student’s t-tests.