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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: Nat Commun. 2013;4:2834. doi: 10.1038/ncomms3834

Figure 4. Akt Signaling is Critical For Polarization in Tsc1Δ/Δ BMDMs.

Figure 4

a. Immunoblot analysis of WT BMDMs pretreated with MK-2206 for 1h and treated with IL-4 for the indicated time points. b and c. WT BMDMs were pretreated with MK-2206 or DMSO for 1h before stimulation with IL-4 for 24h and examination of (b) M2 gene expression (n=3) or (c) urea production (n=4). d. Immunoblot analysis of Tsc1Δ/Δ BMDMs transduced with myr-flag-Akt or empty vector (EV). e and f. Myr-Akt Tsc1Δ/Δ BMDMs and EV Tsc1Δ/Δ BMDMs were stimulated with IL-4 followed 24h later by analysis of (e) M2 gene expression (n=4 representative experiments) or (f) urea production (n=3). g. Cytokine gene expression in myr-Akt Tsc1Δ/Δ BMDMs and EV Tsc1Δ/Δ BMDMs stimulated with LPS for 6 hours (n=3). Graphs are shown as mean ± SEM,*p<0.05, **p<0.01, ***p<0.001. P-values were determined using Student’s t-tests.