TABLE 3.

Effect of ethanol and l-lactate treatment on GHB plasma and brain concentrations at RRR

GHB (600 mg/kg) and ethanol (2.0 g/kg) were administered intravenously, and l-lactate (66 mg/kg plus 302.5 mg/kg per hour) was given intravenously 5 minutes after GHB/ethanol. Animals were euthanized at RRR. One-way analysis of variance followed by Dunnett’s post hoc test was used to determine statistically significant differences compared with GHB plus ethanol. Data from 600 mg/kg alone GHB were used from a previous study (Felmlee et al., 2010b). Data are presented as the mean ± S.D. (n = 3–8).

Treatment Administered	Time of RRR	Cplasma	Cbrain	Brain/Plasma Ratio
	min	μg/ml	μg/ml
GHB	110 ± 17a	352 ± 64a	72.3 ± 12a	0.20 ± 0.03
GHB + ethanol	162 ± 38	178 ± 76	38.7 ± 19	0.21 ± 0.03
GHB + ethanol + l-lactate	93 ± 8a	257 ± 61	37.8 ± 6.3	0.15 ± 0.02a

C_brain, brain GHB concentration at RRR; C_plasma, plasma GHB concentration at RRR.

^aSignificantly different from GHB plus ethanol (P < 0.05).