Fig. 2.

NLRP3 in macrophages and NLRP3, caspase-1, IL-1β, and IL-1α in freshly isolated proximal tubules treated with cisplatin. NLRP3 was not increased in (A) macrophages treated with cisplatin 10 or 50 μM for 6 or 12 hours or (B) proximal tubules treated with cisplatin 10 or 50 μM for 25 minutes. (C) Caspase-1 protein, (D) caspase-1 activity, (E) IL-1β, and (F) IL-1α were increased in proximal tubules treated with cisplatin. β-actin, used as a loading control, was not different between the groups. Representative immunoblots are derived from at least three separate experiments. *P < 0.01 vs. Veh; **P < 0.05 vs. Cis 10.