Regulation of DME and transporter mRNA levels in CAR+/+ and CAR−/− mice following LPS administration. CAR+/+ and CAR−/− mice were i.p. injected with saline or 2 mg/kg LPS, and livers were harvested at 16 hours (n = 5–6 per group). RNA was isolated from the livers, and mRNA levels of phase I enzymes, phase II enzymes, and transporters were determined by real-time PCR analysis, as described earlier. All data are presented as ±S.D. and standardized for cyclophilin mRNA levels. Expression in saline-treated mice was set to 1; fold change after LPS treatment was compared with the saline-treated controls. * and #, Indicate significant difference (P < 0.05) between saline and LPS groups in CAR+/+ mice and CAR−/− mice, respectively, and ‡ indicates basal level differences between CAR+/+ and CAR−/− mice. The experiments were repeated at least thrice.