Fig. 5.

Effects of various inhibitory, monoclonal antibodies against human P450 enzymes on the conversion of metronidazole (100 μM) to 2-hydroxymetronidazole by pooled human liver microsomes. Pooled human liver microsomes (0.05 mg microsomal protein) were preincubated for 15 minutes at room temperature with ascites fluid from mice containing inhibitory monoclonal antibodies against human P450 enzymes or with control IgG followed by incubation with metronidazole (100 μM), as described under Materials and Methods. Volumes of antibodies used followed the manufacturer’s recommendations for maximal P450 inhibition. Each bar represents the mean of duplicate determinations.