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. 2013 Sep;41(9):1642–1650. doi: 10.1124/dmd.113.050930

Fig. 1.

Fig. 1.

Expression and activity of SULT1A1 in nonfatty (control) and diseased human livers. Each data point represents a single tissue (average of determinations) categorized by gender and disease type (n = 20 for nonfatty; n = 13–14 for steatosis; n = 4 for diabetes; n = 21–22 diabetic cirrhosis; and n = 17–22 for alcohol cirrhosis). Tissue from female patients is displayed in red; from male patients, in blue. *Statistically significant differences (P < 0.05) between nonfatty and diseased livers. #Statistically significant differences (P < 0.05) between alcohol cirrhosis and other groups. (A) Enzyme activity was determined by incubating 4 μM pNP with human liver cytosols for 30 minutes in the presence of the 35S-labeled cofactor PAPS. (B) Protein expression of SULT1A1 was quantified by Western blotting analysis in human livers. (C) Messenger RNA expression of SULT1A1 was quantified by branched DNA signal amplification assay (Affymetrix Inc., Santa Clara, CA). (D) Membranes loaded with 40 μg protein and probed with anti-SULT1A1 antibody. Lane numbers refer to sample ID as noted in Supplemental Table 1.