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Graded doses of sox9b MO produce a range of cardiac malformation severity. Wild-type zebrafish embryos were injected with control and sox9b MOs and collected at 96 hpf for brightfield microscopy. Representative brightfield lateral images are shown with anterior to the left. (A) Control MO, (B) sox9b MO (1 nM), (C and D) sox9b MO (2 nM). The Fast Green used as part of the MO injection solution has colored the yolk green (n = 10 fish per treatment).
