TABLE 1.
The P450 content, protein content, and EROD activities of CYP1B1 ortholog bactosomes
Determined by ferrous CO measurements, the bicinchoninic acid method, and resorufin fluorescence, respectively.
P450 Content |
Cytochrome c Reductase Activity |
Protein Content |
EROD Activity |
|
---|---|---|---|---|
pmol/mg protein | nmol/min/mg protein | mg/ml | nmol/min/nmol | |
Human | 690 | 220 | 10.0 | 4.30 ± 0.05 |
Monkey | 632 | 166 | 17.4 | 2.6 ± 0.6 |
Dog | 200 | 1230 | 10.0 | 80.0 ± 10 |
Mouse | 220 | 758 | 9.1 | 6.2 ± 0.3 |
Rat | 36 | 1331 | 15.1 | 2.99 ± 0.01 |
Human (BD)a | 153 | 490 | 6.5 | 15.2 ± 0.9 |
Human CYP1B1 Supersomes from BD Biosciences (San Jose, CA) included as a reference point.