TABLE 3.

Estradiol 4- and 2-hydroxylation by wild-type human, rat, and mutant CYP1B1

Activities are mol product produced per mol P450 per minute.

	4OH E2 Activity	2OH E2 Activity	4OH E2:2OH E2 Ratio
	min−1
Wild-type human	3.1 ± 0.3	0.62 ± 0.06	5.0 ± 0.7
V395L human	0.40 ± 0.03	0.87 ± 0.06	0.46 ± 0.05
L395V rat	0.95 ± 0.02	0.54 ± 0.01	1.78 ± 0.05
Wild-type rat	0.44 ± 0.12	1.17 ± 0.04	0.38 ± 0.10