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. 2013 Dec 15;2013:617569. doi: 10.1155/2013/617569

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Copyright © 2013 Vijayalakshmi Venkatesan et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Apoptosis (TUNEL assay). Apoptosis detected by TUNEL assay shows red (pseudocolor) nuclei (counterstained with DAPI (blue). Mutants showed an increase in (a) apoptosis as compared to Lean and Control and apoptotic index (b) and it is comparable with 24-month-old Controls; (c) represent its negative control. Quantitatively, AI was significantly higher in (d) Mutants compared to Lean and Control and with age. Mean ± SE (n = 6). An asterisk (*) represents significance (P < 0.05 by ANOVA) compared to Control and ($) indicates significance (P < 0.05 by ANOVA) compared to the same phenotype at 1 month.