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. 2013 Dec 15;2013:617569. doi: 10.1155/2013/617569

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Copyright © 2013 Vijayalakshmi Venkatesan et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gene expression (semiquantitative and real-time PCR) mRNA levels for (a) Insulin-1 were increased in Mutants with age as compared to their phenotypes. However (b) Pdx-1 expression was downregulated with age (1 > 6 > 12 months). Gel images have been represented for (a) Insulin-1 and (b) Pdx-1. In line with semiquantitative data, mRNA transcripts were upregulated for (c) Insulin-1 in Mutants with age as compared to their phenotypes. In similar lines (d) Pdx-1 expression was downregulated with age (1 > 6 > 12 months). The values have been represented as 2^∆∆CT against Control. Statistical significance (P < 0.05 by ANOVA) has been represented as compared to Control by an asterisk (*) and as compared to younger age by ($).