FIG. 1.

Inhibition of NF-κB activity prevents MIEP transactivation. Transfection-infection assays were performed in HEL fibroblasts. Cells were cotransfected with the promoter CAT constructs indicated (MIEP, MHC-wt, and MHC-mut) and the IκB-SR or the control construct and then infected with HCMV (MOI, 3 to 5), and CAT assays were performed on the harvested cell lysates. The MHC-wt promoter was used as a positive control, and a mutated MHC promoter with the NF-κB binding sites mutated (MHC-mut) was used as a negative control. Fold induction represents the difference between the percent acetylation of the test sample and that of the vector-alone control. CAT assays were repeated with similar results.