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. Author manuscript; available in PMC: 2014 Sep 25.
Published in final edited form as: J Am Chem Soc. 2013 Sep 17;135(38):10.1021/ja406697t. doi: 10.1021/ja406697t

Figure 4. In vitro synthesis and accumulation of polyketides on CalE8.

Figure 4

PKS-bound intermediates that are assembled on cysteamine-treated CalE8 during reconstitution reactions and released upon alkaline hydrolysis. Shown are HPLC traces (λ = 325 nm, gray; λ = 375 nm, black) of base hydrolysis products from in vitro reactions of CalE8 supplied with no substrate (trace i), 0.5 mM each MalCoA and NADPH (traces ii - iv), or 2 mM each MalCoA and NADPH (trace v). "N" = nonaketide overextension product. Chromatogram intensity scales are identical, allowing for a direct comparison.