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. Author manuscript; available in PMC: 2014 Sep 25.
Published in final edited form as: J Am Chem Soc. 2013 Sep 17;135(38):10.1021/ja406697t. doi: 10.1021/ja406697t

Figure 5. The effect of light exposure and pre-treatment with cysteamine on in vitrosynthesis and accumulation of polyketides on CalE8.

Figure 5

PKS-bound intermediates that are assembled on CalE8 during reconstitution reactions and released upon alkaline hydrolysis. Shown are HPLC traces (λ= 325 nm, gray; λ= 375 nm, black) of base hydrolysis products from in vitro reactions of CalE8 supplied with 0.5 mM each MalSNAc and NAPDH. Chromatogram intensity scales are identical, allowing for a direct comparison.