FIG. 2.
Functional analysis of the boundaries of the PTV-1 IRES. (A) The indicated plasmids were used to program TNT reactions containing [35S]methionine. The products were analyzed by SDS-PAGE and autoradiography. (B and C) The plasmids indicated were used to transfect vTF7-3-infected BHK cells. After 20 h, cell extracts were prepared and analyzed for the expression of CAT and LUC by immunoblotting. (D) The indicated plasmids were assayed as for panels B and C either alone (−) or cotransfected with the plasmid pGEM3Z/J1 (36) that expresses an enterovirus 2A protease (+).