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. 2013 Dec 6;192(1):234–244. doi: 10.4049/jimmunol.1301302

FIGURE 4.

FIGURE 4.

L. monocytogenes induces exocytosis of secondary and primary granules. (A) Neutrophils were incubated for 30 min at 37°C with L. monocytogenes or L. innocua at MOI of 10, washed, and labeled on ice with FITC-conjugated anti-CD66b, anti-CD63, or isotype control Abs. Cells were fixed postlabeling. (B) Neutrophils were incubated for 15 min at 37°C with LLO (0.5–5 nM) or LLOpL (50 nM), fixed, and labeled with FITC-conjugated anti-CD66b, anti-CD63, or isotype control Abs. (A and B) Neutrophil MFI was measured by flow cytometry. Data are expressed as MFI relative to untreated cells. Data from a representative experiment, of three, are shown.