FIGURE 6.

Neutrophil GP inhibit LLO, ALO, and PLY. (A) RAW264.7 macrophages were incubated in medium alone (Control) or with 5 nM LLO ± 6–24% (v/v) GP or CS for 15 min at 37°C. Data are expressed as the mean percentage of maximal LDH release ± SEM of at least three independent experiments, each performed in duplicate. Erythrocytes were incubated with 0.24 nM LLO (B), ALO (C), or PLY (D) and GP (3–24%, v/v) or CS (24%, v/v) for 30 min at 37°C. OD 700 was measured every min for 30 min. Incubation of erythrocytes with PBS or 0.05% Triton X-100 served as negative and positive controls, respectively. (B) Results are the mean ± SEM of at least three independent experiments, performed in duplicate. (C and D) Results are from a representative experiment of two, performed in duplicate. **p < 0.01.